کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174797 961769 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineering of a broad-specificity antibody: Detection of eight fluoroquinolone antibiotics simultaneously
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Engineering of a broad-specificity antibody: Detection of eight fluoroquinolone antibiotics simultaneously
چکیده انگلیسی

Recombinant sarafloxacin-recognizing antibody was engineered with the use of novel fluoroquinolone (FQ) derivatives. A monoclonal FQ antibody, 6H7, was targeted to random mutagenesis to broaden the specificity of the antibody in development of a generic assay for FQ antibiotics. Engineering involved the synthesis of different small-sized FQ molecules to immobilize and detect the mutant antibodies. Selections with labeled FQs resulted in several mutant antibodies with increased affinity or wider specificity toward different FQs. The best characterized mutant antibody was capable of recognizing seven of eight targeted FQs below maximum residue limits set by the European Union. The results are promising in regard to the development of a multiresidue immunoassay for FQs based on a single antibody.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 409, Issue 1, 1 February 2011, Pages 14–21
نویسندگان
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