کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175302 1491337 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch
ترجمه فارسی عنوان
یک روش مبتنی بر پلیمریزاسیون برای ساخت یک پلاسمید حاوی آسیب DNA خوشه بندی شده و عدم تطابق
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Exposure of biological materials to ionizing radiation often induces clustered DNA damage. The mutagenicity of clustered DNA damage can be analyzed with plasmids carrying a clustered DNA damage site, in which the strand bias of a replicating plasmid (i.e., the degree to which each of the two strands of the plasmid are used as the template for replication of the plasmid) can help to clarify how clustered DNA damage enhances the mutagenic potential of comprising lesions. Placement of a mismatch near a clustered DNA damage site can help to determine the strand bias, but present plasmid-based methods do not allow insertion of a mismatch at a given site in the plasmid. Here, we describe a polymerization-based method for constructing a plasmid containing clustered DNA lesions and a mismatch. The presence of a DNA lesion and a mismatch in the plasmid was verified by enzymatic treatment and by determining the relative abundance of the progeny plasmids derived from each of the two strands of the plasmid.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 510, 1 October 2016, Pages 129–135
نویسندگان
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