کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175468 961803 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A method of isolating the collagen (I) α2 chain carboxytelopeptide for species identification in bone fragments
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A method of isolating the collagen (I) α2 chain carboxytelopeptide for species identification in bone fragments
چکیده انگلیسی

We present a novel method for the isolation and analysis of the bone collagen (I) α2 chain carboxytelopeptide as a species biomarker. Conventional methods for the analysis and sequencing of mixtures of proteins and peptides commonly involve using the protease trypsin to cleave proteins present in the sample. However, in the study of collagen, these methods result in very complex mixtures of peptides that are difficult to analyze and the acquired results are not reproducible. Here we use bacterial collagenase (from Clostridium histolyticum) for its ability to cleave the highly unusual Gly-Xaa-Yaa repeating sequence of collagen, where Xaa usually is Pro and Yaa often is Hyp. Followed by a simple isolation step using a reverse phase solid phase extraction cartridge, the α2 (I) chain carboxytelopeptide can be readily analyzed by matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS) and the results can be used to distinguish between different species of origin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 374, Issue 2, 15 March 2008, Pages 325–334
نویسندگان
, , ,