کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175619 961810 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extracting kinetic rate constants from surface plasmon resonance array systems
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Extracting kinetic rate constants from surface plasmon resonance array systems
چکیده انگلیسی

Surface plasmon resonance imaging systems, such as Flexchip from Biacore, are capable of monitoring hundreds of reaction spots simultaneously within a single flow cell. Interpreting the binding kinetics in a large-format flow cell presents a number of potential challenges, including accounting for mass transport effects and spot-to-spot sample depletion. We employed a combination of computer simulations and experimentation to characterize these effects across the spotted array and established that a simple two-compartment model may be used to accurately extract intrinsic rate constants from the array under mass transport-limited conditions. Using antibody systems, we demonstrate that the spot-to-spot variability in the binding kinetics was <9%. We also illustrate the advantage of globally fitting binding data from multiple spots within an array for a system that is mass transport limited.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 373, Issue 1, 1 February 2008, Pages 112–120
نویسندگان
, , , , , , , , ,