کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176186 1491401 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection
ترجمه فارسی عنوان
ساخت یک ایمونسنسور حساس به انسداد بسیار حساس بر پایه اکسید قلع اسیدی برای تشخیص زیست سنجی سرطان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (Rct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the “single frequency impedance” technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 446, 1 February 2014, Pages 9–18
نویسندگان
, ,