کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176194 1491401 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of protein carbamylation in urea solution using ammonium-containing buffers
ترجمه فارسی عنوان
مهار کربامیلاسیون پروتئین در محلول اوره با استفاده از آمونیم حاوی بافر
کلمات کلیدی
کاربامیل کردن، اوره، بافر حاوی آمونیوم، پروتئومیکس، طیف سنجی جرمی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Urea solution is one of the most commonly employed protein denaturants for protease digestion in proteomic studies. However, it has long been recognized that urea solution can cause carbamylation at the N termini of proteins/peptides and at the side chain amino groups of lysine and arginine residues. Protein/peptide carbamylation blocks protease digestion and affects protein identification and quantification in mass spectrometry analysis by blocking peptide amino groups from isotopic/isobaric labeling and changing peptide charge states, retention times, and masses. In addition, protein carbamylation during sample preparation makes it difficult to study in vivo protein carbamylation. In this study, we compared the peptide carbamylation in urea solutions of different buffers and found that ammonium-containing buffers were the most effective buffers to inhibit protein carbamylation in urea solution. The possible mechanism of carbamylation inhibition by ammonium-containing buffers is discussed, and a revised procedure for the protease digestion of proteins in urea and ammonium-containing buffers was developed to facilitate its application in proteomic research.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 446, 1 February 2014, Pages 76–81
نویسندگان
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