کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1176392 | 961848 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit](/preview/png/1176392.png)
چکیده انگلیسی
We report nucleic acid (NA) adsorption isotherms and elution profiles for silica surfaces and use these to design a miniaturized NA purification unit based on solid-phase extraction with silica beads. The procedure is based on a pressure drop equation for flow through a packed bed and allows estimation of key design parameters such as channel dimensions, liquid flow rates, sample volume, and amount of silica needed. The usefulness of this design procedure is demonstrated by applying it to a column-based NA purification device for influenza detection for a case study of Madin–Darby canine kidney cells infected with influenza A virus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 373, Issue 2, 15 February 2008, Pages 253–262
Journal: Analytical Biochemistry - Volume 373, Issue 2, 15 February 2008, Pages 253–262
نویسندگان
Tyson Poeckh, Santiago Lopez, A. Oveta Fuller, Michael J. Solomon, Ronald G. Larson,