کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1176619 961865 2012 11 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of trace human chorionic gonadotropin by using multiwall carbon nanotubes as phosphorescence labeling reagent
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موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of trace human chorionic gonadotropin by using multiwall carbon nanotubes as phosphorescence labeling reagent
چکیده انگلیسی
Taking advantage of the cutting effect of the strong oxidation of benzoyl peroxide [(C6H5CO)2O2] on the end of multiwall carbon nanotubes (MWNTs) to obtain water-soluble multiwall nanotubes (MWNTs') and the spiking effect of polyacrylamide (PA) on the room temperature phosphorescence (RTP) of MWNTs', a new phosphorescent labeling reagent, MWNTs'-PA, has been developed in this study. The product β-AbHCG-MWNTs'-PA obtained by MWNTs'-PA labeling human chorionic gonadotropin-β-subunit three-dimensional core monoclonal antibody (β-AbHCG) not only could maintain good RTP characteristics of MWNTs' but also could take specific immunoreaction with β-HCG to form β-HCG- β-AbHCG-MWNTs'-PA, resulting in the increase of MWNTs' RTP signal. Thus, a new solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of β-HCG has been established. The limits of detection (LODs) of the new method were 0.021 pg spot−1 for the direct way at 447/615 nm (λexmax/λemmax) and 0.016 pg spot−1 for the sandwich way at 447/614 nm (λexmax/λemmax). This sensitive, accurate, and precise method was used to determine β-HCG and diagnose human diseases by the direct way or the sandwich way, with the results coinciding with those obtained by chemiluminescence immunoassay. Meanwhile, the mechanisms of MWNTs' labeling β-AbHCG and determining β-HCG are also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 431, Issue 1, 1 December 2012, Pages 19-29
نویسندگان
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