کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176641 961867 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand
چکیده انگلیسی

De novo sequencing of a full-length monoclonal antibody raised against OX40 ligand is described. Using a combination of overlapping complementary proteolytic and chemical digestions, with analysis by mass spectrometry and Edman degradation, both the heavy and light chains were fully sequenced. Particular attention was paid to those modifications that could be susceptible to degradation in the complementarity determining region and Fc region. An overview of the protocol is described, and suggestions for improvements to aid in such sequencing projects in the future are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 352, Issue 1, 1 May 2006, Pages 77–86
نویسندگان
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