کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176764 961877 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exploring “one-shot” kinetics and small molecule analysis using the ProteOn XPR36 array biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Exploring “one-shot” kinetics and small molecule analysis using the ProteOn XPR36 array biosensor
چکیده انگلیسی

A ProteOn XPR36 parallel array biosensor was used to characterize the binding kinetics of a set of small molecule/enzyme interactions. Using one injection with the ProteOn’s crisscrossing flow path system, we collected response data for six different concentrations of each analyte over six different target protein surfaces. This “one-shot” approach to kinetic analysis significantly improves throughput while generating high-quality data even for low-molecular-mass analytes. We found that the affinities determined for nine sulfonamide-based inhibitors of the enzyme carbonic anhydrase II were highly correlated with the values determined using isothermal titration calorimetry. We also measured the temperature dependence (from 15 to 35 °C) of the kinetics for four of the inhibitor/enzyme interactions. Our results illustrate the potential of this new parallel-processing biosensor to increase the speed of kinetic analysis in drug discovery and expand the applications of real-time protein interaction arrays.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 358, Issue 2, 15 November 2006, Pages 281–288
نویسندگان
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