کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1177028 | 961926 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification of the large ribosomal subunit via its association with the small subunit
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purification of the large ribosomal subunit via its association with the small subunit Purification of the large ribosomal subunit via its association with the small subunit](/preview/png/1177028.png)
چکیده انگلیسی
We have developed an affinity purification of the large ribosomal subunit from Deinococcus radiodurans that exploits its association with FLAG-tagged 30S subunits. Thus, capture is indirect so that no modification of the 50S is required and elution is achieved under mild conditions (low magnesium) that disrupt the association, avoiding the addition of competitor ligands or coelution of common contaminants. Efficient purification of highly pure 50S is achieved, and the chromatography simultaneously sorts the 50S into three classes according to their association status (unassociated, loosely associated, or tightly associated), improving homogeneity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 395, Issue 1, 1 December 2009, Pages 77–85
Journal: Analytical Biochemistry - Volume 395, Issue 1, 1 December 2009, Pages 77–85
نویسندگان
Samuel P. Simons, Thomas J. McLellan, Paul A. Aeed, Richard P. Zaniewski, Charlene R. Desbonnet, Lillian M. Wondrack, Eric S. Marr, Timothy A. Subashi, Thomas J. Dougherty, Zuoyu Xu, Ing-Kae Wang, Peter K. LeMotte, Bruce A. Maguire,