کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1177049 | 961932 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of oxysterols in oxidatively modified low-density lipoprotein by semi-micro high-performance liquid chromatography with electrochemical detection Determination of oxysterols in oxidatively modified low-density lipoprotein by semi-micro high-performance liquid chromatography with electrochemical detection](/preview/png/1177049.png)
A simple method for the determination of oxysterols was developed by semi-micro high-performance liquid chromatography with electrochemical detection (semi-micro HPLC–ECD). Semi-micro HPLC–ECD was established using a C30 microbore column, acetonitrile containing 50 mmol/L LiClO4 as a mobile phase, and an applied potential at +2.8 V versus Ag/AgCl. The current peak height was linearly related to the amount of sterol injected from 12.5 to 250 pmol (r > 0.999) with a relative standard deviation (RSD) of less than 2.9% (n = 6). This method was applied to the determination of seven oxysterols in oxidatively modified low-density lipoprotein (Ox–LDL). Oxysterols were determined with a recovery of more than 78.0% and an RSD of less than 2.9% (n = 6) except for 7-ketocholesterol. 7-Ketocholesterol was determined as a sum of intact 7-ketocholesterol and its degradation product on saponification, cholesta-3,5-dien-7-one, with a recovery of 98.0% and an RSD of 2.5% (n = 6). From these results, the current method enabled the simultaneous determination of seven oxysterols without any derivatization, providing a useful tool for the assessment of oxysterol contents in Ox–LDL.
Journal: Analytical Biochemistry - Volume 393, Issue 2, 15 October 2009, Pages 222–228