کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177375 961995 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metal particle-enhanced fluorescent immunoassays on metal mirrors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Metal particle-enhanced fluorescent immunoassays on metal mirrors
چکیده انگلیسی

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 363, Issue 2, 15 April 2007, Pages 239–245
نویسندگان
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