کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177603 962036 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition assay of β-hematin formation initiated by lecithin for screening new antimalarial drugs
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Inhibition assay of β-hematin formation initiated by lecithin for screening new antimalarial drugs
چکیده انگلیسی

Measurement of heme crystallization provides a tool for screening new antimalarial drugs. Current assays for heme crystallization have employed initiators such as thermo, histidine-rich proteins, and lipids extracted from parasites and infected plasma. These initiators are unnatural or require laborious steps to prepare. In this study, we used a commercially available lipid, lecithin, a kind of phospholipid containing about 50% unsaturated fatty acids, as an initiator for heme crystal (β-hematin) formation. We demonstrated that the inhibition of lecithin-based β-hematin formation by antimalarial drugs is highly correlated with the preformed β-hematin-based method. In addition, the lecithin-based assay is sensitive and convenient for large-scale screening of new novel antimalarials. We also indicated that dimethyl sulfoxide is an ideal solvent for preparation of heme stock solution, which is stable and can be used for 1 month.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 349, Issue 2, 15 February 2006, Pages 292–296
نویسندگان
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