کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1192865 | 1492302 | 2011 | 13 صفحه PDF | دانلود رایگان |
PSD does not usually generate a complete series of y-type ions, particularly at high mass, and this is a limitation for de novo sequencing algorithms. It is demonstrated that b2 and b3 ions can be used to help assign high mass xN−2 and xN−3 fragments that are found in vacuum ultraviolet (VUV) photofragmentation experiments. In addition, vN-type ion fragments with side chain loss from the N-terminal residue often enable confirmation of N-terminal amino acids. Libraries containing several thousand peptides were examined using photodissociation in a MALDI-TOF/TOF instrument. 1345 photodissociation spectra with a high S/N ratio were interpreted.
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► We study the VUV photodissociation of peptides found in synthetic libraries.
► The propensities for forming various unusual high-energy fragment ions are examined.
► The potential for using high-energy fragment ions in de novo sequencing is discussed.
Journal: International Journal of Mass Spectrometry - Volume 308, Issues 2–3, 1 December 2011, Pages 142–154