کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1194830 | 1492346 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method](/preview/png/1194830.png)
The kinetic method has been applied for determination of d-Phe/l-Phe enantiomeric ratio. Discrimination of enantiomers was inferred from product ion mass spectra of trimeric cluster ions containing the analyte (l,d-Phe), Cu2+ as a central metal and l-Trp as a chiral reference ligand. Unsatisfactory quantitative results achieved on an ion trap were rationalized by high-resolution mass spectrometry. The formation of Mg2+-containing cluster isobaric to trimeric cluster [Cu(l-Trp)2Phe]+ was observed. Interference like this was identified as a possible reason for deterioration of quantitative low-resolution mass spectrometric analyses of real-world samples based on the kinetic method. Cation-exchanger was used for easy removal of magnesium from a sample and improvement of quantitation.Chiral dependence of formation of the Cu2+-containing trimeric cluster was also observed. Heterochiral diastereoisomeric ions were created less effectively.
Journal: International Journal of Mass Spectrometry - Volume 280, Issues 1–3, 1 February 2009, Pages 213–217