کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
12099 778 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pattern stability under cell culture conditions—A comparative study of patterning methods based on PLL-g-PEG background passivation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Pattern stability under cell culture conditions—A comparative study of patterning methods based on PLL-g-PEG background passivation
چکیده انگلیسی

Despite the rapidly increasing number of publications on the fabrication and use of micro-patterns for cell studies, comparatively little is know about the long-term stability of such patterns under cell culture conditions. Here, we report on the long-term stability of cellular patterns created by three different patterning techniques: selective molecular assembly patterning, micro-contact printing and molecular assembly patterning by lift-off. We demonstrate that although all three techniques were combined with the same background passivation chemistry based on assembly of a PEG-graft copolymer, there are considerable differences in the long-term stability between the three different pattern types under cell culture conditions. Our results suggest that these differences are not cell-dependent but are due to different (substrate-dependent) interactions between the patterned substrate, the passivating molecule and the serum containing cellular medium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 27, Issue 12, April 2006, Pages 2534–2541
نویسندگان
, , , , ,