کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1251272 | 1496036 | 2015 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development and validation of analytical method for Naftopidil in human plasma by LC–MS/MS Development and validation of analytical method for Naftopidil in human plasma by LC–MS/MS](/preview/png/1251272.png)
A highly sensitive and simple high-performance liquid chromatographic–tandem mass spectrometric (LC–MS-MS) assay is developed and validated for the quantification of Naftopidil in human plasma. Naftopidil is extracted from human plasma by methyl tertiary butyl ether and analyzed using a reversed-phase gradient elution on a discovery C 18 5 μ (50 × 4.6) column. A methanol: 2 mM ammonium formate (90:10) as mobile phase, is used and detection was performed by MS using electrospray ionization in positive mode. Propranolol is used as the internal standard. The lower limits of quantification are 0.495 ng/mL. The calibration curves are linear over the concentration range of 0.495–200.577 ng/mL of plasma for each analyte. This novel LC–MS-MS method shows satisfactory accuracy and precision and is sufficiently sensitive for the performance of pharmacokinetic studies in humans.
Journal: Arabian Journal of Chemistry - Volume 8, Issue 5, September 2015, Pages 648–654