کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251374 | 970905 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of Catalytic Domain of Protein Tyrosine Phosphatase 1B and Preparation of Its Polyclonal Antibody*
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
This study focuses on the expression of human protein tyrosine phosphatase 1B(PTP1B) catalytic domain (ÎPTP1B) and preparation of polyclonal antibody against ÎPTP1B. ÎPTP1B gene was PCR amplified with the cDNA of human PTP1B as the template, and cloned into the pT7 expression vector. The recombinant pT7-ÎPTP1B was expressed in E. coli Rosetta (DE3) host cells and purified. The antiserum was prepared by immunizing rabbit with purified recombinant ÎPTP1B. The polyclonal antibody against ÎPTP1B was purified by PVDF immobilized antigen affinity chromatography. ÎPTP1B was correctly cloned, expressed, and purified as confirmed by PCR, DNA sequence analysis, SDS-PAGE western blotting, and HPLC. The titer and sensitivity of the antibody were 1: 2500(volume ratio) and 0.1 ng, respectively. This study provides an important basis for further studying the biological function of PTP1B and its relationship with human diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Research in Chinese Universities - Volume 23, Issue 2, March 2007, Pages 204-207
Journal: Chemical Research in Chinese Universities - Volume 23, Issue 2, March 2007, Pages 204-207
نویسندگان
Dong-lei SHI, Hong-bo DONG, Zhi-cheng ZHU, Mei SUN, Ji-quan SU, Xing-yi ZHANG, Yue-chi XU, Xue-qi FU,