کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251576 | 970918 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and Characterization of a Proteolytic Enzyme from Fig Latex
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant(Km) for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Research in Chinese Universities - Volume 24, Issue 3, May 2008, Pages 348-352
Journal: Chemical Research in Chinese Universities - Volume 24, Issue 3, May 2008, Pages 348-352
نویسندگان
Lu HUANG, He-zhi QU, Lei ZHANG, Shan-shan DU, Shuo YANG, Dong-yun HAO, Xiao-ping WANG,