کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1253997 | 971323 | 2015 | 5 صفحه PDF | دانلود رایگان |
Single cell analysis is of great significance to understand the physiological activity of organisms. Microfluidic droplet is an ideal analytical platform for single-cell analysis. We developed a microfluidic droplet splitting system integrated with a flow-focusing structure and multi-step splitting structures to form 8-line droplets and encapsulate single cells in the droplets. Droplet generation frequency reached 1021 Hz with the aqueous phase flow rate of 1 μL/min and the oil phase flow rate of 15 μL/min. Relative standard deviation of the droplet size was less than 5% in a single channel, while less than 6% in all the 8 channels. The system was used for encapsulating human whole blood cells. A single-cell encapsulation efficiency of 31% was obtained with the blood cell concentration of 2.5 × 104 cells/μL, and the multicellular droplet percentage was only 1.3%. The multi-step droplet splitting system for single cell encapsulation featured simple structure and high throughput.
We developed a simple microfluidic droplet splitting system to encapsulate single cells in the droplets with an encapsulation efficiency of 31% and a droplet generation frequency of 1021 Hz.Figure optionsDownload as PowerPoint slide
Journal: Chinese Chemical Letters - Volume 26, Issue 12, December 2015, Pages 1450–1454