Development of a triple channel detection probe for hydrogen peroxide

The rapid and reliable measurement of hydrogen peroxide (H2O2) is imperative for many areas of technology, including pharmaceutical, clinical, food industry and environmental applications. In this work, a novel multifunctional complex, [Ru(bpy)2(luminol-bpy)](PF6)2 (bpy: 2,2′-bipyridine), was designed and synthesized by incorporating a Ru(II) complex with a luminal group. In the presence of horseradish peroxidase (HRP), reaction of [Ru(bpy)2(luminol-bpy)]2+ with H2O2 can be monitored by three sensing channels including photoluminescence (PL), chemiluminiscence (CL) and eletrochemiluminiscence (ECL). The quantitative assays for H2O2 in aqueous solutions using [Ru(bpy)2(Luminal-bpy)](PF6)2 as a probe were established with PL, ECL and CL signal output modes, respectively.

A novel complex, [Ru(bpy)2(luminol-bpy)]2+, can monitor H2O2 by three sensing channels including photoluminescence (PL), chemiluminiscence (CL) and eletrochemiluminiscence (ECL).Figure optionsDownload as PowerPoint slide