کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1255278 971416 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Design of thiol-containing amino acids for native chemical ligation at non-Cys sites
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Design of thiol-containing amino acids for native chemical ligation at non-Cys sites
چکیده انگلیسی

Protein chemical synthesis usually relies on the use of native chemical ligation that couples peptide thioester with a Cys-peptide. A limitation of this method is the difficulty of finding an appropriate Cys ligation site in many synthetic targets. To overcome this problem, the ligation–desulfurization approach has been developed. This approach involves the use of a thiol-containing amino acid as the ligation partner. After the sequence assembly is completed, the thiol group is removed through a desulfurization reaction to generate the standard amino acids. Currently this strategy has been applied to the ligations at a number of amino acids including Ala, Phe, Val, Lys, Thr, Leu, Pro and Gln. The present article reviews the design and synthesis of these thiol-containing amino acids for native chemical ligation at non-Cys sites.

Recent advances in design and synthesis of thiol-containing amino acids in ligation–desulfurization strategy for native chemical ligation at non-Cys sites.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Chemical Letters - Volume 24, Issue 4, April 2013, Pages 265–269
نویسندگان
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