Structural characterization of 1β,11α-dihydroxycanrenone biotrans-formed from canrenone by Aspergillus ochraceus SIT34205

Canrenone (1) was biotransformed into 11α-hydroxycanrenone (2) and a main byproduct (3) by Aspergillus ochraceus SIT34205. Compound 3 was separated and purified using silica gel column chromatography, and its structure was characterized via MS and NMR methods. These results indicated that 3 was 1β,11α-dihydroxycanrenone and the product of further hydroxylation of 2. Thus, investigating the structure and synthesis of 3 may be a promising method to improve the efficiency and purity of 2.