کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1257375 971559 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast separation of hen egg white protein with a phosphorylcholine type zwitterionic ion chromatography stationary phase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Fast separation of hen egg white protein with a phosphorylcholine type zwitterionic ion chromatography stationary phase
چکیده انگلیسی

In this work, a kind of preparation method of zwitterionic ion chromatography (ZIC) stationary phase modified with phosphorylcholine (PC) was obtained by hydrolyzing after bonding phosphorylcholine dichloride to diol-silica to better explore the characteristics of the PC groups as ZIC stationary phase ligand in simultaneous separation of acidic proteins and basic proteins. The results showed that two kinds of acidic proteins and three kinds of basic proteins can be separated completely, meanwhile, hen egg white was separated and purified and three kinds of egg white components ovalbumin, G2 ovoglobulin and ovotransfemin proteins were separated completely by one single step on PC-ZIC column, the purity of all proteins reached above 95%. PC-ZIC stationary phase was successfully improved with better separation capacity and selectivity than previously reported in this paper.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Chemical Letters - Volume 23, Issue 12, December 2012, Pages 1389–1392
نویسندگان
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