Development of a monolithic polymer pipette for solid-phase extraction of liquiritigenin in rat plasma

A monolithic polymer column with mixed-mode interaction was prepared by in situ polymerization in a 1000 μL pipette. Two kinds of monomers, butyl methacrylate (BMA) and 2-(dimethylamino)ethyl methacrylate (DMAM) were applied to constructing the mixed-mode interaction of monolithic polymer column. Its solid-phase extraction properties for liquiritigenin (LQG) were evaluated by high performance liquid chromatography (HPLC) with a gradient elution procedure. After the extraction procedure was optimized, the maximum binding capacity and extraction recovery following the optimal extraction procedure were investigated. Calibration curve was expressed as A = 65.9C + 4.53 (r2 = 0.998) with a linear range of 0.151–1.80 μg/mL. The experimental results indicate that the monolithic polymer pipette presents good extraction efficiency for LQG. It can be envisaged that the developed monolithic polymer pipette possesses the potential for its application to the enrichment of other flavonoids compounds being similar to the structure of LQG.