Determination of levels of adenosine phosphates in blood by ion chromatography

A new method was developed for simultaneous determination of levels of AMP, ADP and ATP in blood by anion-exchange chromatography. The three adenosine phosphates were separated by Dionex IonPac AG18 (50 mm × 4 mm) guard column and IonPac AS18 (250 mm × 4 mm) analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits (S/N = 3) of AMP, ADP and ATP were 38, 47, 108 ng/L, respectively. The relative standard deviations of retention time, peak area and peak height were all less than 1.87% and a good linear relationship was obtained. This method was applied to analyze human blood samples.