Electrochemical studies of chloroperoxidase on poly-l-lysine film modified GC electrode

Poly-l-lysine (PLL) was first electrodeposited onto the surface of a glassy carbon (GC) electrode. The PLL modified electrode was used to immobilize chloroperoxidase (CPO) via 1-[(3-dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDC). The electrochemical behaviors of immobilized CPO on PLL/GC electrode were investigated by cyclic voltammetry (CV). The CV results obtained showed that CPO was successfully immobilized on the PLL/GC electrode and a fast direct electron transfer between CPO and PLL–GC electrode was achieved with a formal redox potential of −0.23 V vs. SCE. The CPO–PLL/GC modified electrode showed a good catalytic activity for electrocatalytical reduction of O2, promising for a broad range of CPO-catalyzed transformations.