Determination of sodium hyaluronate with some basic bisphenylnaphthylmethane dyes by resonance Rayleigh scattering method

In an acetic acid–sodium acetate buffer solution of pH 3.6–6.8, a compound complex was formed between sodium hyaluronate (abbreviated as SH) and some basic bisphenylnaphthylmethane dyes, leading to a great enhancement of the intensity of resonance Rayleigh scattering (RRS) and giving a new RRS spectrum, with its maximum scattering peak near 280 nm. It was also found that the intensity of RRS was directly proportional to the concentration of SH near the range between 0 and 3.0 mg/L. Based on these facts, a sensitive method for the determination of SH has been established. The method had good selectivity, and has been used for the determination of total amounts of SH in samples with satisfactory results. For the NB–SH system, the detection limit of SH was down to 13.7 ng/mL.