کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1265664 1496873 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of platinum nanoparticles on cell death induced by ultrasound in human lymphoma U937 cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Effect of platinum nanoparticles on cell death induced by ultrasound in human lymphoma U937 cells
چکیده انگلیسی


• First study to determine the dual effects of platinum nanoparticles (Pt-NPs).
• Combination of Pt-NPs and US might be effective in cancer eradication.
• Pt-NPs suppress US-induced apoptosis and inhibit autophagy resulting in increased cell killing.
• Pt-NPs suppress US induced OH radical formation in aqueous solution.

In this study, we report on the potential use of platinum nanoparticles (Pt-NPs), a superoxide dismutase (SOD)/catalase mimetic antioxidant, in combination with 1 MHz ultrasound (US) at an intensity of 0.4 W/cm2, 10% duty factor, 100 Hz PRF, for 2 min. Apoptosis induction was assessed by DNA fragmentation assay, cell cycle analysis and Annexin V-FITC/PI staining. Cell killing was confirmed by cell counting and microscopic examination. The mitochondrial and Ca2+-dependent pathways were investigated. Caspase-8 expression and autophagy-related proteins were detected by spectrophotometry and western blot analysis, respectively. Intracellular reactive oxygen species (ROS) elevation was detected by flow cytometry, while extracellular free radical formation was assessed by electron paramagnetic resonance spin trapping spectrometry. The results showed that Pt-NPs exerted differential effects depending on their internalization. Pt-NPs functioned as potent free radical scavengers when added immediately before sonication while pre-treatment with Pt-NPs suppressed the induction of apoptosis as well as autophagy (AP), and resulted in enhanced cell killing. Dead cells displayed the features of pyknosis. The exact mode of cell death is still unclear. In conclusion, the results indicate that US-induced AP may contribute to cell survival post sonication. To our knowledge this is the first study to discuss autophagy as a pro-survival pathway in the context of US. The combination of Pt-NPs and US might be effective in cancer eradication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics Sonochemistry - Volume 31, July 2016, Pages 206–215
نویسندگان
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