کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1267247 1496927 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of base-pair sequence on electrochemically driven denaturation
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
The effect of base-pair sequence on electrochemically driven denaturation
چکیده انگلیسی

Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, Em) directly correlates with the calculated nearest neighbour and experimental melting temperatures; Tm, for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.


► Immobilised dsDNA can be denatured by scanning the electrode potential cathodic.
► Changing the DNA sequence changes the electrochemical melting potential.
► The potential at which the dsDNA denatures correlates with the melting temperature.
► Nearest neighbour melting models can be used to predict the melting potential.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 85, June 2012, Pages 7–13
نویسندگان
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