Nanoband array electrode as a platform for high sensitivity enzyme-based glucose biosensing

• Glucose oxidase was covalently attached to thiol-modified nanoband array electrodes.
• The response of the sensor to hydrogen peroxide and glucose was monitored electrochemically.
• Comparing with a conventional electrode, a thousand-fold improvement in sensitivity and wide linear range was obtained.
• The superior performance of the nanoband sensor can be attributed to the nanoband array architecture.
• The nanoband structure allows for efficient diffusion of substrate to the electrode and provides low background currents.

We describe a novel glucose biosensor based on a nanoband array electrode design, manufactured using standard semiconductor processing techniques, and bio-modified with glucose oxidase immobilized at the nanoband electrode surface. The nanoband array architecture allows for efficient diffusion of glucose and oxygen to the electrode, resulting in a thousand-fold improvement in sensitivity and wide linear range compared to a conventional electrode. The electrode constitutes a robust and manufacturable sensing platform.