کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1269593 972458 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel amperometric method for antioxidant activity determination using DPPH free radical
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
A novel amperometric method for antioxidant activity determination using DPPH free radical
چکیده انگلیسی

A new method for the determination of antioxidant activity based on the amperometric reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) at the glassy carbon electrode is proposed. All experiments were done in three-electrode electrochemical cell at 140 mV vs. Hg2Cl2 | 3 M KCl using ethanolic solution (φ = 40%) and 0.033 M KCl in 0.033 M phosphate buffer, pH = 7.4. The linear range obtained for Trolox in 100 μM DPPH ethanol–water solution was up to 30 μM, with a limit of detection of 0.05 μM.The developed method was applied for the evaluation of antioxidant activity of some water or ethanol soluble pure compounds of antioxidants and of the samples of tea, wine and some other beverages. The good correlation of measurements (R2 = 0.9993) expressed as Trolox equivalent was obtained between the proposed amperometric method and classic spectroscopic method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 68, Issue 2, May 2006, Pages 175–180
نویسندگان
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