Electroporation-mediated genetic vaccination for antigen mapping: Application to Plasmodium falciparum VAR2CSA protein

Genetic vaccination, consisting in delivering a genetically engineered plasmid DNA by a non-viral vector or technique into a tissue, is currently of great interest. New delivery technique including DNA transfer by electroporation recently greatly improved the potency of this concept. Because it avoids the step of producing a recombinant protein, it is particularly of use in studying the immunogenic properties of large proteins. Here we describe the use of electroporation mediated DNA immunization to identify important protective epitopes from the large VAR2CSA protein from Plasmodium falciparum implicated in the pathology of placental malaria. Immunizing mice and rabbit with DNA plasmids encoding different fragments of VAR2CSA leads to high titer antisera. Moreover an N-terminal region of the protein was found to induce protective functional antibodies.

► Genetic vaccination was used to rapidly identify antigenic fragments.
► Plasmid electroporation for genetic vaccination against Plasmodium falciparum protein.
► A VAR2CSA protein fragment against placental-malaria erythrocyte adhesion.