Microbial analysis of anodic biofilm in a microbial fuel cell using slaughterhouse wastewater

► A two-chambered microbial fuel cell generates electricity from slaughterhouse wastewater. ► Fuel cell voltage-time profile correlates with production of anolyte VFA content. ► Biofilm developed under 100 Ω load yields increased power compared to that developed under 1 MΩ. ► Bacterial community analysis indicates external load induces bacterial diversity.