Effects of chronic kidney disease on blood cells membrane properties

Chronic kidney disease (CKD) is progressive loss of renal function associated among others with increased intracellular calcium concentration. The purpose of this study was to identify the effects of CKD on cell membrane properties such as human red blood cell Ca2 + ATPase activity, lymphocyte plasma membrane P2X7 receptor expression and function. This could help us in elucidating the origin of increased calcium concentration in blood cells. We found out Ca2 + ATPase activity is decreased in early stage CKD patients resulting in altered calcium removal from cytoplasm. By means of flow cytometry we assessed that P2X7 receptor expression on lymphocyte membrane is 1.5 fold increased for CKD patients. Moreover, we detected an increased uptake of ethidium bromide through this receptor in CKD at basal conditions. It means CKD lymphocyte membranes contain more receptors which are more permeable thus allowing increased calcium influx from extracellular milieu. Finally, we can state alterations in blood cell membranes are closely linked to CKD and may be responsible for intracellular calcium accumulation.

► Membrane alterations in early stages chronic kidney disease.
► Increased calcium influx through P2X7 receptor and increased P2X7 receptor expression.
► Decreased plasma membrane calcium ATPase activity.
► Multifactorial reasons of increased calcium level in peripheral mononuclear cells.