کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1314898 | 975990 | 2011 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Fluorous solvent for cell culture Fluorous solvent for cell culture](/preview/png/1314898.png)
Incubation of mouse melanoma B16 cells in fluorous solvents with low boiling point such as perfluoromethylcyclohexane, 1,1,1,3,3,3-hexafluoro-2-propanol, ethylpentafluoropropionate resulted in cell death. However, cells lived up to 2 days in fluorous alcohols such as 2,2,3,3,4,4,5,5-octafluoro-1-pentanol and 3,3,4,4,5,5,6,6,6-nonafluoro-1-hexanol with relatively higher fluorine content. Remarkably, cells survived deprived of nutrition up to 4 days when incubated in 2,2,3,3,4,4,5,5,6,6,6-undecafluoro-1-hexanol or in 2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptanol that have the most number of fluorine atoms (11 and 12, respectively) among the perfluoroalkyl alcohols used, and with boiling points of 128 °C and 169 °C, respectively.
Mouse melanoma B16 cells were cultured in the presence of various fluorous solvents. Remarkably, cells survived deprived of nutrition up to 4 days when incubated in 2,2,3,3,4,4,5,5,6,6,6-undecafluoro-1-hexanol and in 2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptanol.Figure optionsDownload as PowerPoint slideHighlights
► Mouse melanoma B16 cells were incubated in various fluorous solvents.
► Deprived of nutrition, B16 cells survived up to 4 days in perfluoroalcohols.
► The number of fluorine atoms of fluorous solvent affected B16 cell viability.
► Hydrophobicity of fluorous solvents accounts for survival of cells.
Journal: Journal of Fluorine Chemistry - Volume 132, Issue 11, November 2011, Pages 978–981