کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1351341 | 1500407 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNA barcoding of sunn pest adult parasitoids using cytochrome c oxidase subunit I (COI) DNA barcoding of sunn pest adult parasitoids using cytochrome c oxidase subunit I (COI)](/preview/png/1351341.png)
• Sunn pest adult parasitoid species are potential biological control agents of the most important pest of cereals.
• DNA barcoding was used in the identification of sunn pest adult parasitoid species.
• Four parasitoids and sixteen haplotypes were analyzed using COI gene.
• DNA barcoding approaches may prove useful for monitoring the success of biological control programs.
In this study, DNA barcoding was used in the identification of potential biological control agents of sunn pest adult parasitoid species, including Eliozeta helluo (F.), Phasia subcoleoptrata (L.), Ectophasia crassipennis (F.) and Elomyia lateralis (Meig). DNA analyses were assessed by sequencing cytochrome c oxidase subunit I (COI) gene. The obtained sequences were analyzed in terms of nucleotide composition, nucleotide pair frequency and haplotype diversity. Genetic divergence among haplotypes was estimated by constructing genetic distance matrix using DNA sequence variations, by Kimura 2-parameter model. Variable sites and average variations of the sequenced 603 base pair long DNA fragment were calculated. All COI barcodes were matched with reference sequences of expected species according to morphological identification. Neighbor-joining tree was drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at species level. The evolutionary history inferred using the UPGMA method indicated two distinct mitochondrial haplotype lineages. The genetic variation between sunn pest adult parasitoids will be useful in sunn pest management, regulatory and environmental applications.
Journal: Biochemical Systematics and Ecology - Volume 59, April 2015, Pages 70–77