کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1353767 1500400 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of Odontamblyopus lacepedii via morphology and DNA barcoding
ترجمه فارسی عنوان
شناسایی Odontamblyopus lacepedii از طریق مورفولوژی و بارکدسازی DNA
کلمات کلیدی
Odontamblyopus lacepedii؛ شخصیت های مورفولوژیکی؛ سیتوکروم اکسیداز زیر واحد I (COI)؛ بارکدسازی DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• A more detailed morphological investigation of Odontamblyopus lacepedii was made than that of before.
• Genetic analysis using related species supported Odontamblyopus lacepedii identification.
• Combination with morphometric characters and DNA barcoding, Odontamblyopus lacepedii can be identified precisely.

To evaluate taxonomic and genetic variation in Odontamblyopus lacepedii collected in East Asia, morphometric characters and molecular analysis were described. The body was ribbon-like. The body and head were grayish-purple with no embedded scales. Eyes were rudimentary, but distinct, covered by skin. The dorsal and anal fins were continuous with the caudal fin. The edges of the dorsal, anal, and caudal fins were dark chocolate-brown. Genetic analysis of four species revealed higher mean A + T (55.4%) than G + C (44.6%). Twenty-two haplotype sequences were identified, containing 22 haplotypes, 131 variable sites and 122 parsimony-informative sites, with haplotype and nucleotide diversity differing among species. The intraspecies genetic distances ranged from 0.2% to 0.5% (average 0.3%) and interspecies differences from 1.1% to 17.6% (average 14.6%), respectively, indicating that these four species of Amblyopinae can be accurately identified. The NJ tree constructed using COI fragments based on 22 haplotypes was composed of two main branches: the upper branch comprising O. lacepedii and O. sp. showing a close relationship, with Trypauchen vagina and Ctenotrypauchen microcephalus clustering on the second branch. Thus, the results validated the identification of O. lacepedii at the morphological and genetic level.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Systematics and Ecology - Volume 66, June 2016, Pages 281–285
نویسندگان
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