کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1353865 1500408 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular traits to elucidate the ancestry of Helianthus x multiflorus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Molecular traits to elucidate the ancestry of Helianthus x multiflorus
چکیده انگلیسی


• The hybridization background of Helianthus x multiflorus (3n) has been elucidated.
• Helianthus annuus and Helianthus decapetalus (2n) were revealed as parental species.
• Comparison of cpDNA provided additional information on the maternal parent species.

The triploid perennial Helianthus x multiflorus L. is a beloved garden ornamental, but its phylogenetic origin has long been a source of discussion. Sequence comparison of genes involved in the biosynthesis of the sesquiterpene lactones was used to identify the common sunflower Helianthus annuus L. and the diploid taxon Helianthus decapetalus L. as the most likely parental combination in the origin of the triploid hybrid species. A pair of deletions (22 and 26 bp in length) in an intron part of the germacrene A synthase 1 gene differentiated the diploid from the tetraploid karyotype of H. decapetalus. The same deletion was found in one of the two alleles (299 bp in length) in H. x multiflorus, whereas a second allele (350 bp in length) showed a very high similarity with the sequence found in H. annuus. Quantification of the two amplification products in H. x multiflorus showed a higher concentration of the larger amplicon thus suggesting that H. annuus has contributed an unreduced genome to the hybrid. Comparison of variable regions of the cpDNA showed highest identity of H. x multiflorus with cpDNA sequences of the diploid H. decapetalus. This suggests that H. decapetalus served as the maternal parent in a cross with H. annuus to produce H. x multiflorus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Systematics and Ecology - Volume 58, February 2015, Pages 51–58
نویسندگان
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