Changes in plasma osmolality and Na+/K+ ATPase activity of juvenile obscure puffer Takifugu obscurus following salinity challenge

• Plasma osmolality of juvenile obscure puffer transferred to 30‰ salinity increased.
• Na+/K+ ATPase in gill at various salinities firstly increased and then decreased.
• Fluctuation trends of Na+/K+ ATPase in kidney were similar as that in gill.
• Modified Gaussian model provided accurate fit for fluctuation trend in Na+/K+ ATPase.

The osmoregulatory capabilities of 6-month-old juvenile obscure puffer Takifugu obscurus, transferred directly from fresh water to different salinities (0‰, freshwater control; 10‰; 20‰ and 30‰), were studied over an 8-day period. After transfer, plasma osmolality of the fish at 30‰ was significantly higher than those at all other salinities throughout the experiment. The Na+/K+ ATPase activity in the gills of the fish treated with various salinities increased significantly, peaking at 48 h, then decreased gradually to the control level at 192 h. Similar fluctuation trends of the Na+/K+ ATPase activity were observed in the kidneys. Modified Gaussian model provided accurate fits for the time-course changes in the Na+/K+ ATPase activities after abrupt salinity challenge. The results demonstrated that obscure puffer has strong capacity to tolerate abrupt salinity changes and can osmoregulate well over a wide range of salinities even in juvenile stage.