Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA

• Resonance light scattering (RLS) was developed to identify the target of oridonin.
• The interaction of oridonin with DNA leads to great increase of RLS intensity.
• The binging mode of oridonin with DNA was confirmed.
• The conclusion was validated by UV, fluorescence, fluorescence polarization and AFM.
• The assay possesses the merits of simple, rapidness and cheapness.

In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI.

A sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering technique.Figure optionsDownload as PowerPoint slide