کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1394909 1501094 2016 9 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
The induction of apoptosis in HepG-2 cells by ruthenium(II) complexes through an intrinsic ROS-mediated mitochondrial dysfunction pathway
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موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
The induction of apoptosis in HepG-2 cells by ruthenium(II) complexes through an intrinsic ROS-mediated mitochondrial dysfunction pathway
چکیده انگلیسی


• Four new ruthenium (II) complexes [Ru(N–N)2(dhbn)](ClO4)2 were synthesized.
• The cytotoxicity of these complexes was evaluated by MTT method.
• The apoptosis was investigated with AO/EB and Hoechst 33258 staining methods.
• The cellular uptake and DNA damage were investigated with fluorescence microscope.
• Investigation of the expression of proteins involved in apoptosis pathway was performed.

Four new ruthenium(II) polypyridyl complexes [Ru(N–N)2(dhbn)](ClO4)2 (N–N = dmb: 4,4′-dimethyl-2,2′-bipyridine 1; bpy = 2,2′-bipyridine 2; phen = 1,10-phenanthroline 3; dmp = 2,9-dimethyl-1,10-phenanthroline 4) were synthesized and characterized. The cytotoxicity in vitro of the ligand and complexes toward HepG-2, HeLa, MG-63 and A549 were assayed by MTT method. The IC50 values of the complexes against the above cells range from 17.7 ± 1.1 to 45.1 ± 2.8 μM. The cytotoxic activity of the complexes against HepG-2 cells follows the order of 4 > 2 > 3 > 1. Ligand shows no cytotoxic activity against the selected cell lines. Cellular uptake, apoptosis, comet assay, reactive oxygen species, mitochondrial membrane potential, cell cycle arrest, and the expression of proteins involved in apoptosis pathway induced by the complexes were investigated. The results indicate that complexes 1–4 induce apoptosis in HepG-2 cells through an intrinsic ROS-mediated mitochondrial dysfunction pathway.

Four new Ru(II) complexes [Ru(N–N)2(dhbn)]2+ were synthesized and characterized. The in vitro cytotoxicity, apoptosis, cellular uptake, ROS, mitochondrial membrane potential, cell cycle arrest and western blot analysis were investigated.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Medicinal Chemistry - Volume 122, 21 October 2016, Pages 118–126
نویسندگان
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