کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
144077 | 438921 | 2014 | 6 صفحه PDF | دانلود رایگان |
• The sheet-like Hap particles were examined for the adsorption behavior of proteins.
• The amounts of adsorbed proteins, especially for BSA, could be controlled optionally.
• The BSA molecules could be completely separated from BSA–LSZ mixed solution.
The protein adsorption behavior onto sheet-like calcium hydroxyapatite Ca10(PO4)6(OH)2 (Hap) particles produced by microreactor was examined by using typical acidic bovine serum albumin (BSA) and basic lysozyme (LSZ). Since the sheet-like Hap particles were highly growth through b- (or a-) and c-axes, the particles had larger fraction of bc (or ac ) crystal face. The saturated amounts of adsorbed BSA (nsBSA) for the sheet-like Hap nanoparticles were increased with increase in the particle size; i.e., nsBSA showed a good linear relationship with the total surface area of bc and ac faces of each particle. This result strongly suggested that the sheet-like Hap particles progressed in b- and c-axes with large fraction of C sites on bc and ac particle faces and exhibit a high selective adsorption of BSA. On the contrary, very few LSZ molecules adsorbed onto sheet-like Hap particles. Hence the present study developed that the amounts of adsorbed protein, especially for BSA, can be controlled optimally by altering the size of sheet-like Hap particles. It was also found that the sheet-like Hap particles can be applied to separate completely BSA molecules from BSA–LSZ mixed solution.
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Journal: Advanced Powder Technology - Volume 25, Issue 1, January 2014, Pages 354–359