کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1441986 | 1509429 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polypyrrole colloidal nanospheres as an effective fluorescent sensing platform for DNA detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
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چکیده انگلیسی
In the present study, we demonstrate the first use of polypyrrole colloidal nanospheres (PPyCNPs) as an effective sensing platform for fluorescence-enhanced DNA detection by the following two steps: (1) PPyCNP adsorbs and quenches a dye-labeled single-stranded DNA (ssDNA) probe; (2) The subsequent hybridization of the probe with its target produces a double-stranded DNA (dsDNA) which detaches from PPyCNP, leading to recovery of dye fluorescence. The present system shows a detection limit as low as 1 nM and has a high selectivity down to single-base mismatch.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Synthetic Metals - Volume 161, Issues 15–16, August 2011, Pages 1766–1770
Journal: Synthetic Metals - Volume 161, Issues 15–16, August 2011, Pages 1766–1770
نویسندگان
Wenbo Lu, Yonglan Luo, Guohui Chang, Xiaoyun Qin, Fang Liao, Xuping Sun,