Lipase immobilized on nanostructured cerium oxide thin film coated on transparent conducting oxide electrode for butyrin sensing

► Developed lipase/nano-CeO2/TCO biosensor for tributyrin sensing. ► Fluorine doped cadmium oxide (CdO:F) TCO film acts as working electrode. ► CeO2 film acts as nanointerface between lipase and the working TCO electrode. ► The biosensor showed a sharp response time of 5 s with a shelf life period of 6 weeks. ► The biosensor exhibited excellent linearity (0.33-1.98 mM) with a LOD of 2 μM.