Study of electron spin resonance and viscosity for hemoglobin polymer after arsenic trioxide and gamma irradiation treatment

The present work aimed to study the intensity of electron spin resonance (ESR) of hemoglobin rat's polymer and changes in rheological properties (viscosity). This study included five groups: control, irradiation with single dose of 5 Gy gamma irradiation, intraperitoneal injection with single dose of arsenic trioxide (As2O3) 10 mg/kg body weight, As2O3 followed by 5 Gy and 5 Gy followed by As2O3. The results of ESR spectroscopy indicated that the intensity increases significantly for As2O3 and non-significantly with 5 Gy of gamma radiation compared with control, which indicated an increase in the number of free radicals. Injection with As2O3 after and before 5 Gy resulted in non-significant decrease which given rise to a decrease in the number of free radicals. there was significant decrease in viscosity for 5 Gy at different shear rates (11.3–375 sec−1), viscosity for As2O3 injection group showed non-significant decrease, and non-significant increase in case of 5 Gy followed by As2O3 injection, significant decrease at high shear rate in case of 5 Gy followed by As2O3. These results concluded that 5 Gy followed by As2O3 showed some sort of repair in the function of rats hemoglobin rather than injection with As2O3 and 5 Gy both individually.