Bioleaching of sphalerite sample from Kooshk lead-zinc tailing dam

The zinc extraction from Kooshk lead-zinc tailing dam's sample was investigated by bioleaching method. The Kooshk lead-zinc deposit/mine is located in Yazd province, Iran, and its tailing dam contains about 3.64% zinc, 0.97% lead and 24.18% iron. Experiments were designed and carried out by a mixed culture of mesophile bacteria as well as a mixed culture of moderate thermophile strain in the shake flasks. The results indicated that, more than 90% of sphalerite was dissolved during 14 d, while without bacteria, 44% of Zn was merely extracted. In addition, some experiments were performed in the absence of the bacterial medium (9K). The results of these experiments indicated significant difference in Zn extraction with and without 9K until the 10th day of bioleaching, but after that the Zn extraction was improved and the same extraction was achieved at the end of bioleaching tests. This improvement can be attributed to the increase of the number of bacteria or Fe3+ concentration at the last days of leaching. Zn extraction kinetics of moderate thermophile bacteria was significantly higher than that of the mesophile, therefore sphalerite was successfully dissolved in preference to the pyrite using moderate thermophile bacteria in a lower redox potential.