Isolation, characterization and extraction of mer gene of Hg2+ resisting strain D2

Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence, growth, minimal inhibitory concentrations (MICs) of metals, antibiotic susceptibility and mer gene were conducted. The strain D2 can grow in the medium containing 60 mg/L mercury ion. It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa, and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance. The mer RT gene sequence was amplified from chromosome. Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.