Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99–125 lipase via adsorption

Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99–125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5 mg·g− 1 support and enzyme activity of 2631.6 U·(g support)− 1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

Magnetically modified palygorskite composites comprised of γ-Fe2O3 nanoparticles dispersed in a clay matrix were prepared. Magnetic measurement reveals that the saturated magnetization of magnetically modified palygorskite composites was 13.4 emu·g− 1. Lipase immobilized on magnetically modified palygorskite composites can be easily separated and recovered with magnet assistance outside the reaction system by magnet assistance. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase. The immobilized lipase maintains 75.6% of its activity even after 8 cycles of use.Figure optionsDownload as PowerPoint slide